Spectrophotometer calibration ensures accurate measurements and is done using a reference solution. Different types of spectrophotometers require different calibration methods, and warm-up time should be considered. Calibration can be done in the lab or by the manufacturer if needed.
Spectrophotometer calibration is a process in which a scientific instrument known as a spectrophotometer is calibrated to confirm that it is functioning properly. This is important, as it ensures that the measurements obtained with the meter are accurate. The procedure varies slightly for different tools, with most manufacturers providing a detailed calibration guide in their owner’s manual so people know how to properly calibrate the equipment. When this process is performed, the person performing it should note in the logbook attached to the equipment and in the test notes, so people know when the device was last calibrated and handled, and by whom.
A spectrophotometer is capable of both transmitting and receiving light. The device is used to analyze test material samples by passing light through the sample and reading the intensity of the wavelengths. Different samples affect light in different ways, allowing a researcher or technician to learn more about the materials in the test sample by observing how the light behaves as it passes through the sample. Calibration of the spectrophotometer is required to confirm that the results are accurate.
In spectrophotometer calibration, a reference solution is used to zero the equipment. This solution provides a base or zero reading. The device is calibrated by placing the reference solution inside the spectrophotometer, resetting the settings and starting the instrument. Then, samples of an actual test material can be subjected to spectrophotometry with confidence that the machine has been calibrated and is functioning properly.
In a single beam spectrophotometer, a single beam of light is generated and the device must be recalibrated for each use. In a dual-beam spectrophotometer, beams can be sent through a test sample and a reference sample simultaneously to generate two sets of results that can be used for reference and calibration. Either way, spectrophotometer calibration can be done in the lab by someone who works with the machine. If the machine has serious problems, it can be sent to the manufacturer for maintenance, repair and possible replacement.
For a spectrophotometer to function properly, it needs to warm up before use. Many devices take about 10 minutes to warm up. It is important to avoid calibrating the spectrophotometer during the warm-up phase as this will invalidate the settings. It is also important to be aware that for some types of wavelengths, special filters and accessories may be required for the device to function.
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