Hemoglobin electrophoresis is a blood test that identifies different types of hemoglobin and their ratios in a patient’s blood. It is used to diagnose diseases and evaluate treatment effectiveness, and can identify abnormal hemoglobins such as sickle cell disease. The test uses protein electrophoresis to distinguish between different types of hemoglobin based on their electrical […]
Electrophoresis separates charged particles, commonly used in biology to separate DNA or protein molecules. Various procedures are used based on the type and size of molecules. Gels are commonly used as a support medium, with agarose for DNA and acrylamide for proteins. Capillary and 2-D electrophoresis are advanced techniques. The electrophoresis process uses an electric […]
Capillary electrophoresis separates molecules based on size and electrical charge. An electric field is applied, causing molecules to move at different speeds. UV light or fluorescent detection is used to locate molecules. It has applications in genetics, food science, and medical research. In biochemistry, capillary electrophoresis is a technique used to separate molecules based on […]
DNA gel electrophoresis separates molecules based on their electrical charge. It is used in forensics, genetics, and biochemistry. Different techniques, such as Southern blotting and zymography, can be used to detect specific nucleotide sequences or biological functions. Gel electrophoresis is commonly used in forensics to determine correlations with available suspects and in genetics to determine […]
RNA gel electrophoresis separates RNA molecules by size and charge using a gelatinous sheet. RNA must be purified and denatured before being added to the gel. Separating fragments by size can be useful for comparing samples. In biochemistry, RNA gel electrophoresis is a common method used to analyze the biological molecule called ribonucleic acid (RNA). […]
DNA electrophoresis separates DNA fragments by size using an electric field and agarose gel. Ethidium bromide is used to visualize the bands formed by DNA fragments of different sizes. This process is used in various scientific studies and is useful for replicating, separating, or examining genes on DNA strands. DNA electrophoresis is the process of […]
Gel electrophoresis separates molecules of different sizes by applying an electric current to a gel matrix containing DNA, RNA, or protein samples. Samples are added to wells and separated based on size. This technique is used in various fields of biological research. In gel electrophoresis, an electric current is applied to a gel matrix that […]
2D gel electrophoresis separates protein mixtures along two different axes, often used for complicated or thick protein mixes. The technique employs protein separation based on two different protein characteristics, unlike one-dimensional gel electrophoresis. Gel electrophoresis separates molecules by size across a porous substrate, often using molecular charges or mass as a feature. Polyacrylamide gels are […]
DNA electrophoresis separates DNA molecules by length using an electric field and gel. Restriction enzymes cut DNA, which is mixed with a dye or radioisotope and separated by electrophoresis. Gel electrophoresis can identify genetic traits, isolate DNA strands, and create genetic profiles for identification. The process has been in development since the 1930s and was […]
Protein electrophoresis separates and analyzes proteins in fluid samples using an electric charge. It can diagnose and monitor conditions such as liver disease, anemia, and cancer. It is used on urine, blood serum, and cerebrospinal fluid samples. Protein electrophoresis is a medical procedure used to separate and analyze the proteins present in a fluid sample. […]