Proteins have different pH values due to their amino acids. The isoelectric point (pI) is when a protein has no net charge. Isoelectric focusing separates proteins with known pI from a mixture. Glycosylation can affect pI. Proteins can be sequenced and analyzed for purity. Proteins are made up of chains of amino acids, each of […]
Isoelectric focusing separates molecules based on their electrical charge. Proteins have a net charge determined by the pH level of their environment. Manipulating the protein’s environment with an electric field causes particles to move towards the cathode or anode, allowing for separation. Isoelectric focusing (IEF), also called electrofocusing, is a means of separating molecules based […]
