DNA sequencing is a process of determining the sequence of nucleotide bases in a DNA molecule. There are several methods, with dideoxynucleotide sequencing being the most popular. The process involves extracting DNA from cells, sequencing it, sorting it by size, and analyzing it with a computer. DNA sequencing is a collection of scientific methods for […]
Bisulfite sequencing is a sensitive method of analyzing DNA methylation, which can be used to determine active regions of a genome and identify gene-rich areas. However, it requires high salt concentrations and may result in false positive calls. It cannot be used on living people but is useful for analyzing clinical samples. Bisulfite sequencing is […]
Ribosomal RNA sequencing determines the sequence content of ribosomes, which build proteins in cells. The process involves duplicating and identifying small fragments of RNA. This data can aid in bacterial identification and drug design by targeting unique subsequences in bacterial ribosomal RNA. Ribosomal ribonucleic acid (RNA) sequencing is the process of determining the sequence content […]
RNA sequencing determines the nucleotide sequence in RNA, which contains genetic information used to make proteins or perform specific functions. Scientists sequence DNA more often, but RNA sequencing is important to discover the precise genetic code and examine unused segments of genetic information. Reverse transcription is often necessary, but researchers aim to develop direct sequencing […]
Shotgun sequencing breaks DNA into small fragments, clones, sequences and assembles them using computer analysis. Developed by Craig Venter, it competed with the Human Genome Project’s map-based sequencing. Whole genome sequencing bypasses mapping and starts with DNA sequencing, resulting in a contig. Shotgun sequencing is now the preferred method for genome sequencing. Shotgun sequencing is […]
