What is ANOVA?

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ANOVA is a statistical test used to analyze data by comparing multiple samples or groups. It examines differences between and within samples to determine if there is a significant relationship. ANOVA has four assumptions and tests the null hypothesis that there is no difference between mean values. The F-ratio is used to determine the rejection area for the null hypothesis. If there is a significant difference between all groups, it can be concluded.

When doing research, it sometimes becomes necessary to analyze data by comparing more than two samples or groups. One type of inferential statistical test, the analysis of variance (ANOVA), allows you to examine multiple samples simultaneously in order to determine whether there is a significant relationship between them. The reasoning is identical to t-tests, only analysis of variance includes independent variables from two or more samples. The differences between samples and the difference within a sample are determined. ANOVA is based on four assumptions: the level of measurement, the sampling method, the distribution of the population, and the homogeneity of the variance.

In order to determine whether the differences are significant, ANOVA deals with the differences between and within samples, which is referred to as the variance. ANOVA can find out whether the variance is greater between samples than between sample members. If this is true, the differences are considered significant.

Taking an ANOVA test implies that you accept certain assumptions. The first is that the independent random sampling method is used and the choice of sample members from a single population does not influence the choice of members from subsequent populations. Dependent variables are primarily measured at the interval ratio level; however, it is possible to apply analysis of variance to ordinal-level measures. It can be assumed that the population is normally distributed, although this is not verifiable, and the population variances are the same, meaning that the populations are homogeneous.

The research hypothesis assumes that at least one mean is different from the others, but the different means are not identified as higher or lower. It is only expected that there is a difference. ANOVA tests the null hypothesis, meaning that there is no difference between all mean values, such that A = B = C. This requires setting the alpha, referring to the level of probability in which the null hypothesis will be rejected.

The F-ratio is a test statistic used specifically for analysis of variance, since the F-score shows where the rejection area for the null hypothesis begins. Developed by statistician Ronald Fisher, the formula for F is as follows: F = between-group variance estimate (MSB) divided by the within-group variance estimate (MSW), so that F = MSB/MSW. Each of the variance estimates has two parts: the sum of squares (SSB and SSW) and the degrees of freedom (df). Using Statistical Tables for Biological, Agricultural and Medical Research, alpha can be set up and based on this, and the null hypothesis of no difference can be rejected. It can be concluded that there is a significant difference between all groups if this is the case.




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