A UV spectrophotometer measures the interaction between radiation and matter, identifying electronic transitions within the electromagnetic spectrum. It can measure visible light and the near-to-visible range of the ultraviolet and infrared spectrum ranges. The device can identify the exact levels of compounds within a particular spectrum sample and is used extensively in the printing industry to ensure correct color placement. The Beer-Lambert law affects the analytical data of the spectrophotometer, and a bi-spectral fluorescent mechanism is required to detect fluorescence.
A UV spectrophotometer is a device used to study the interaction between radiation and matter with regards to the wavelength of photons. Specifically, it measures visible light and the near-to-visible range of the ultraviolet and infrared spectrum ranges. The device allows the user to identify electronic transitions within various regions of the electromagnetic spectrum.
UV light is most easily measured by a spectrophotometer when it is in the region of 400 to 700 nanometers (nm) to quantify and determine the characteristics of color perception. Essentially, the device allows scientists to take measurements of the ability of the human eye and mind to isolate specific wavelengths that define colors. The study of colors within the human perceptual range is known as colorimetry.
Traditionally, a spectrophotometer cannot detect fluorescence. This requires an add-on known as a bi-spectral fluorescent mechanism. Without this capability, color images are difficult to handle properly, particularly if the color contains some sort of fluorescence.
A UV spectrophotometer is set up in two different formats known as d/8, a spherical scan, and 0/45, a linear scan. Both designations are oriented towards identifying the specific geometric pattern of the light source, as well as the environment of the object. It also takes into account what an observer would naturally see when looking at the coloration.
An advantage of this device is the fact that it can identify the exact levels of compounds within a particular spectrum sample. For example, if it analyzes a photograph, it should be able to identify the different color components of each section of the image. Each color and color saturation are identifiable.
The Beer-Lambert law states that different colors are also affected by the materials through which light travels. This will have a profound influence on the analytical data of the spectrophotometer. For this reason, light absorption on specific materials can have divergent results.
The printing industry uses UV spectrophotometers extensively to ensure that the correct color is placed on a product. Ink production relies on technology to ensure that the base ink composition produces the correct color when placed on paper or boxes. The device also continues to monitor results when actual printing occurs. By taking readings across the 10 to 20 nm spectrum in visible light, the spectrophotometer can identify the output of the spectral reflectance curve, radiation reflected from an object’s surface.
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